Questions and Answers

Question Set Nine

(a) You should previously have observed that the zinc atom has one face exposed to the solvent in the absence of the substrate. What is this space now occupied by? Specifically, what atom does the zinc coordinate and why? An InsightII model may help you with this question.

(b) What amino acid residues in the active site are induced to fit the substrate? An InsightII model showing CPA with and without substrate is available, as well as an InsightII model which superimposes two CPA molecules with and without substrate.

(c) Are there any obvious changes to the shape or conformation of the protein outside the active site following binding of the substrate. If so, identify the region! This Insight II model will help you this question.

End of Question Set Nine Back to Exercises